RT Journal Article
SR Electronic
T1 The effect of siRNA on invasion capability of small cell lung cancer
JF European Respiratory Journal
JO Eur Respir J
FD European Respiratory Society
SP P1229
VO 40
IS Suppl 56
A1 Tao Chen
A1 Jian An Huang
A1 Chuan Yong Mu
A1 Yuan Zeng Gu
YR 2012
UL http://erj.ersjournals.com/content/40/Suppl_56/P1229.abstract
AB Objective To study the inhibitory effect of CXCR4-targeted small interference RNA on invasion capability of NCI-H446 in vitro.Methods To design chemical synthesis of CXCR4-specific siRNA based on the target sequence for CXCR4 cDNA with NCI-H446 cells transfected with siRNA. To detect the expression of CXCR4 by RT-PCR and Western Blot. Invasion capability of NCI-H446 cells in vitro was evaluated by transwell chamber model and the proliferation capability was determined by CCK-8 assay.Results After transfected with CXCR4-siRNA, the expression of CXCR4 mRNA and protein was down-regulated significantly. The invasion capability of cells in vitro decreased compared with the empty liposome group. The penetrating number in CXCR4-siRNA transfected group was 32.3±3.8 VS 62.1±8.2(P<0.001).There was no effect on cell proliferation after transfection of CXCR4 siRNA on NCI-H446 cells.Conclusion CXCR4 siRNA effectively down-regulated the expression of CXCR4 gene and decreased invasion capability of NCI-H446 cells in vitro.