PT  - JOURNAL ARTICLE
AU  - Tao Chen
AU  - Jian An Huang
AU  - Chuan Yong Mu
AU  - Yuan Zeng Gu
TI  - The effect of siRNA on invasion capability of small cell lung cancer
DP  - 2012 Sep 01
TA  - European Respiratory Journal
PG  - P1229
VI  - 40
IP  - Suppl 56
4099  - http://erj.ersjournals.com/content/40/Suppl_56/P1229.short
4100  - http://erj.ersjournals.com/content/40/Suppl_56/P1229.full
SO  - Eur Respir J2012 Sep 01; 40
AB  - Objective To study the inhibitory effect of CXCR4-targeted small interference RNA on invasion capability of NCI-H446 in vitro.Methods To design chemical synthesis of CXCR4-specific siRNA based on the target sequence for CXCR4 cDNA with NCI-H446 cells transfected with siRNA. To detect the expression of CXCR4 by RT-PCR and Western Blot. Invasion capability of NCI-H446 cells in vitro was evaluated by transwell chamber model and the proliferation capability was determined by CCK-8 assay.Results After transfected with CXCR4-siRNA, the expression of CXCR4 mRNA and protein was down-regulated significantly. The invasion capability of cells in vitro decreased compared with the empty liposome group. The penetrating number in CXCR4-siRNA transfected group was 32.3±3.8 VS 62.1±8.2(P<0.001).There was no effect on cell proliferation after transfection of CXCR4 siRNA on NCI-H446 cells.Conclusion CXCR4 siRNA effectively down-regulated the expression of CXCR4 gene and decreased invasion capability of NCI-H446 cells in vitro.