RT Journal Article
SR Electronic
T1 Neutrophil phagocytic activity in AECOPD
JF European Respiratory Journal
JO Eur Respir J
FD European Respiratory Society
SP p738
VO 38
IS Suppl 55
A1 Lavinskiene, Simona
A1 Vaitkus, Mindaugas
A1 Bieksiene, Kristina
A1 Jeroch, Jolanta
A1 Sakalauskas, Raimundas
YR 2011
UL http://erj.ersjournals.com/content/38/Suppl_55/p738.abstract
AB Background: Neutrophils are the major inflammatory cells in pathogenesis of chronic obstructive pulmonary disease (COPD). It is known that phagocytic activity of these cells is decreased during stable COPD (SCOPD). Otherwise, it is unknown how neutrophils act in acute exacerbation of COPD (AECOPD).Aim: To investigate neutrophils phagocytic activity in AECOPD.Methods: 10 patients with SCOPD, 7 with AECOPD and 10 healthy individuals (HI) were studied. Neutrophils were isolated from peripheral blood by high-density gradient Ficoll. Phagocytosis was investigated using different concentrations of FITC labeled S. aureus bacteria (1-167 bacteria/neutrophil (b/n)). Phagocytic activity was analyzed using flow cytometer assessing the cellular immunofluorescence intensity in neutrophil population.Results: Neutrophil phagocytic activity was decreased in patients with AECOPD in comparison with SCOPD and HI. The highest phagocytosis was obtained using 167 b/n concentration in AECOPD and SCOPD groups (6.52±2.5-fold and 8.84±1.8-fold), but phagocytic activity was lower compared with HI (13.5±2.2-fold) (p<0.05).Conclusion: Our results shows that neutrophils phagocytosis is decreased during AECOPD.