TY - JOUR T1 - The specificity of a new skin test – Diaskintest (recombinant protein CFP10-ESAT6) in patients with sarcoidosis and non-tuberculous pulmonary diseases JF - European Respiratory Journal JO - Eur Respir J VL - 38 IS - Suppl 55 SP - p294 AU - Natalya Makarova AU - Ludmila Slogotskaya AU - Elena Kupavtseva AU - Sergey Borisov Y1 - 2011/09/01 UR - http://erj.ersjournals.com/content/38/Suppl_55/p294.abstract N2 - Background: DIASKINTEST (DST) is a recombinant protein combination of CFP10-ESAT6 for intradermal injection and does not cause delayed-type hypersensitivity reactions in the BCG vaccinated.Objectives and methods: We have used DST intradermally in a dose of 0.2 mg in 0.1 ml in 179 patients aged 18-69 yrs.: 62 patients with morphologically identified sarcoidosis, 11 active tuberculosis (TB) patients and 106 patients with other nonspecific inflammatory lung diseases. In 72 hours we measured the diameter of induration and hyperemia in mm. Positive response is evaluated as an any size induration, negative - no induration and hyperemiaResults: Among sarcoidosis patients we saw no reaction to DST – the negative results were in all 62 cases, and specificity of DST in this group was 100% [95%CI 99.9%-100%]. In 106 patients with nontuberculous inflammatory lung diseases we had 8 positive result (2 patients with asthma, 2 with nonspecific pneumonia, 2 with lung cancer, 2 with COPD); specificity of the method in this group was 92,5% [95%CI 87.4-97.5%]. 2 of 11 TB patients had negative response to DST (1 patient with TB and hepatitis C and 1 with TB and alcoholism), so sensitivity is 81,8% for TB cases [95%CI 58.9%-100%]. In our previous research we had similar results using IFN-g release assay (IGRA) in response of blood cells to ESAT-6 ex vivo, but in the clinical practice we see that the IGRA is more laborious method.Conclusion: DST has high specificity in sarcoidosis patients and in patients with other nonspecific inflammatory lung diseases. DST may be used as an additional test for the differential diagnosis with TB in unclear cases. ER -