RT Journal Article
SR Electronic
T1 LSC 2011 Abstract: Effect of inhaled apocynin on reactive oxygen species concentrations in exhaled breath condensate of asthmatics
JF European Respiratory Journal
JO Eur Respir J
FD European Respiratory Society
SP p3265
VO 38
IS Suppl 55
A1 Stefanska, Joanna
A1 Sarniak, Agata
A1 Wlodarczyk, Anna
A1 Sokolowska, Milena
A1 Doniec, Zbigniew
A1 Nowak, Dariusz
A1 Pawliczak, Rafal
YR 2011
UL http://erj.ersjournals.com/content/38/Suppl_55/p3265.abstract
AB Reactive oxygen species (ROS) have a strong impact on homeostasis and are thought to play an important role in inflammation in asthma. The sources of oxidative stress in patients with chronic inflammatory lung diseases derive mainly from increased amounts of ROS and reactive nitrogen species (NOS), generated by airway cells. Apocynin is and agent which blocks NADPH oxidase - the enzyme, responsible for ROS production. The anti-inflammatory activity of apocynin has been demonstrated in a variety of cells and animal models of inflammation. Therefore, considering apocynin activities, we investigated the effect of nebulized apocynin in 14 nonsmoking asthmatics, in placebo-controlled, cross-over design study. Effects of apocynin have been checked 30, 60 and 120 minutes after nebulization by collecting exhaled breath condensate (EBC) samples. Additionally, we investigated safety parameters.Apocynin significantly decreased H2O2 concentration in EBC in comparison to placebo after 60 and 120 min. (0.29 μM vs. 0.44 μM, and 0.27 μM vs. 0.4 μM, respectively). Moreover, apocynin significantly reduced NO2- concentration 30 and 60 min after nebulization (2.75 μM vs. 4.65 μM, and 2.5 μM vs. 4.05 μM, respectively) in comparison to placebo. Finally, apocynin caused a significant decrease of NO3- concentration in EBC after 60 and 120 min after administration, comparing to placebo (5.34 μM vs. 8.2 μM (60 min), and 5.3 μM vs. 8 μM, (120 min) respectively). No influence of apocynin on safety parameters, and no adverse effects has been observed.These data suggest that using apocynin might be a promising solution to alleviate inflammatory process, and probably, symptoms of inflammatory diseases.