%0 Journal Article %A Milena Baptistella Grotta %A Edson Antunes %A Dalize Squebola %A Adyleia Dalbo Contrera Toro %A Maria Angela Ribeiro %A Silvia Barros Mazon %A Jose Dirceu Ribeiro %T Chemotaxis and adhesion from peripheral eosinophils in atopic asthmatic children with and without obesity %D 2011 %J European Respiratory Journal %P p4280 %V 38 %N Suppl 55 %X Background: The prevalence of obesity and asthma has increased over the past several decades. Recent investigations suggest relationship between asthma and obesity in many studies, but the mechanisms are unclear. The aim of this study was evaluate chemotaxis and adhesion of eosinophils in atopic asthmatic children and adolescents with and without obesity.Method: 32 obese asthmatic and non obese asthmatic and 5 healthy volunteers were included. Peripheral blood was collected and eosinophils were purified using a Percoll gradient followed by immunomagnetic cell separator. Chemotaxis was performed with microchemotaxis chamber in triplicate with spontaneous chemeotaxis (MEM), eotaxin, platelet activating factor (PAF) and regulated on activation, normal T cell expressed and secreted (RANTES). The measurement was done by optical microscope count. The adhesion was performed by fibronectin plates in triplicate with MEM and eotaxin. Eosinophilic adhesion was calculated by comparison between absorbance of unknown samples with the standard curve.Results: In spontaneous chemotaxis, with eotaxin and PAF, there was significant difference in the number of eosinophils between obese asthmatic, non obese and healthy volunteers (p<0.05). RANTES increased between asthmatic obese and healthy volunteers groups (p<0.05). Spontaneous adhesion and with eotaxin increased the adhesion of eosinophils between obese asthmatic and healthy volunteers (p<0.05).Conclusion: This is the first study that demonstrated higher eosinophilic activity (chemotaxis and adhesion) in obese atopic asthmatic children than non obese asthmatic and healthy volunteers. %U