PT  - JOURNAL ARTICLE
AU  - Adriana Rozy
AU  - Marta Kedzior
AU  - Joanna Chorostowska-Wynimko
AU  - Paulina Jagus
AU  - Adam Szpechcinski
AU  - Ewa Skrzypczak-Jankun
AU  - Jerzy Jankun
TI  - Diverse effects of PAI-1 proteins on lung and prostate cancer cell invasion
DP  - 2011 Sep 01
TA  - European Respiratory Journal
PG  - p3816
VI  - 38
IP  - Suppl 55
4099  - http://erj.ersjournals.com/content/38/Suppl_55/p3816.short
4100  - http://erj.ersjournals.com/content/38/Suppl_55/p3816.full
SO  - Eur Respir J2011 Sep 01; 38
AB  - Acquisition of ability to uncontrolled migration is one of the fundamental properties of cancer cells, enabling them to infiltrate tissues and metastasize. PAI-1 is the major physiological inhibitor of urokinase (uPA), which plays a key role in migration and invasion of tumor cells. The aim of present study was to analyze the impact of increasing concentrations of PAI-1 mutated forms: VLHL PAI-1 with very long half-life time, Vn neg PAI-1 - devoid of affinity towards vitronectin and wPAI -1 on lung (A549, H1299) and prostate (LNCaP, DU145) cancer cells invasive activity. Selected cell lines are characterized by different (normal and high) urokinase production.No effect of PAI-1 proteins on invasiveness of lung cancer cells was observed, while dose-dependent significant inhibition was demonstrated in both prostate cancer lines (DU145 and LNCaP) cultured with VLHL PAI-1 (respectively p<0,05 and p<0,01) and Vn PAI-1 neg (p<0,05). Not surprisingly wPAI-1 significantly stimulated prostate cancer cells invasiveness in all concentrations.PAI-1 inhibitory effect on prostate cancer invasive activity is associated with anti-proteinase activity. Lung cancer cells invasiveness regulation seems not to be PAI-1-urokinase regulated.