%0 Journal Article %A Lamia Yacoubi %A Raja Serairi %A Lotfi Rabaoui %A Mohamed Hedi Hamdaoui %A Nadia Kourda %A Fatma Tritar %A Sonia Maalej %A Leila Douik %A Saloua Ben Khamsa %T Anti-oxidative and anti-inflammatory effects of trigonella foenum graecum (fenugreek) seed extract in experimental pulmonary fibrosis %D 2011 %J European Respiratory Journal %P p676 %V 38 %N Suppl 55 %X Background: Oxidative stress participated in the pathogenesis of interstitial lung diseases. This prospective study is the first experimental work that evaluated the effect of Fenugreek (FG) and its phenolylic extract on oxidative stress and lung fibrosis in experimental model.Methods: A single dose of Bleomycine was injected intratracheally in forty male wistar rats (4mg/kg). Two days after inducing lung injuries, animals were randomly divided in four groups of 10 rats: G1, G2, G3 and G4. G1received FG Seed Polyphenol Extract at a dose of 200 mg kg by daily gavage.G2 received FG seeds powder mixed with food at levels of up to 20%. G3 received an equal volume of water (6.5 ml kg/, daily gavage). G4 did not receive any treatment. Two weeks after inducing lung fibrosis, rats were sacrificed and we evaluated stress biomarkers (TAS:Total Antioxdant Status, Malon DiAldehyde:MDA) in serum and inflammatory index, fibrosis score and TGF beta density in injured lung.Results: Comparatively to control groups G3 and G4, ANOVA statiscal study, demonstrated that FG Seed Polyphenols Extracts ameliorated TAS and reduced inflammtory index and TGFβ density in G1 and seeds powder of FG reduced MDA a marker of oxidant stress and inflammatory score in G2 (p <0,05).However, Ashcroft semi-quantitative assessing of fibrosis score has not revealed significant differences between the four groups (p>0,05).Conclusion: This study demonstrated that FG ' has potent antioxidant and anti-inflammatory activities but has not an anti-fibrotic effect confirming that besides inflammation, other factors probably interfere in the pathogenesis of pulmonary fibrosis. %U