RT Journal Article
SR Electronic
T1 Proteomic analysis of antioxidant proteins in bronchoalveolar lavage of patients with pulmonary Langerhans-cell histiocytosis
JF European Respiratory Journal
JO Eur Respir J
FD European Respiratory Society
SP p3809
VO 38
IS Suppl 55
A1 Paola Rottoli
A1 Claudia Landi
A1 Elena Bargagli
A1 Antje Prasse
A1 Barbara Magi
A1 Maria Grazia Perari
A1 Joachim Muller-Quernheim
A1 Luca Bini
YR 2011
UL http://erj.ersjournals.com/content/38/Suppl_55/p3809.abstract
AB Pulmonary Langerhans-cell histiocytosis (PLCH) is a tabacco smoke-related diffuse lung disease of unknown etiopathogenesis. Oxidative-mediated lung injury has been documented in patients with PLCH. In this study we analyzed by 2-dimensional electrophoresis, BAL protein expression of antioxidant proteins in a group of patients with PLCH, a group of healthy smokers and a group of no-smoker controls in order to better understand the potential role of oxidant/antioxidant balance in the disease pathogenesis. Among the differentially expressed proteins identified by us, the proteins implicated in the antioxidant defense where thioredoxin (THIO), ALBU, ceruloplasmin (CERU) and glutathione peroxidase 3 (GPX3). THIO was a protein identified from a spot down-regulated in PLCH versus smoker controls (p<0.01). It plays a protective role against cigarette smoke-induced lung oxidative damage and against Th2-driven airway inflammation. CERU isoform 1 and 2 were significantly upregulated in PLCH patients compared to no-smoker controls (p<0.01) while CERU isoform 3 was upregulated in patients than smoker controls (p<0.05). GPX3 was absent in 2-DE gels of patients with PLCH and it was significantly over-expressed in smokers than no-smoker controls. In conclusion antioxidant defense could be involved in PLCH pathogenesis being the expression of antioxidant proteins significantly different in BAL of patients with respect to smoker or no smoker controls.