PT - JOURNAL ARTICLE AU - Paola Rottoli AU - Claudia Landi AU - Elena Bargagli AU - Antje Prasse AU - Barbara Magi AU - Maria Grazia Perari AU - Joachim Muller-Quernheim AU - Luca Bini TI - Proteomic analysis of antioxidant proteins in bronchoalveolar lavage of patients with pulmonary Langerhans-cell histiocytosis DP - 2011 Sep 01 TA - European Respiratory Journal PG - p3809 VI - 38 IP - Suppl 55 4099 - http://erj.ersjournals.com/content/38/Suppl_55/p3809.short 4100 - http://erj.ersjournals.com/content/38/Suppl_55/p3809.full SO - Eur Respir J2011 Sep 01; 38 AB - Pulmonary Langerhans-cell histiocytosis (PLCH) is a tabacco smoke-related diffuse lung disease of unknown etiopathogenesis. Oxidative-mediated lung injury has been documented in patients with PLCH. In this study we analyzed by 2-dimensional electrophoresis, BAL protein expression of antioxidant proteins in a group of patients with PLCH, a group of healthy smokers and a group of no-smoker controls in order to better understand the potential role of oxidant/antioxidant balance in the disease pathogenesis. Among the differentially expressed proteins identified by us, the proteins implicated in the antioxidant defense where thioredoxin (THIO), ALBU, ceruloplasmin (CERU) and glutathione peroxidase 3 (GPX3). THIO was a protein identified from a spot down-regulated in PLCH versus smoker controls (p<0.01). It plays a protective role against cigarette smoke-induced lung oxidative damage and against Th2-driven airway inflammation. CERU isoform 1 and 2 were significantly upregulated in PLCH patients compared to no-smoker controls (p<0.01) while CERU isoform 3 was upregulated in patients than smoker controls (p<0.05). GPX3 was absent in 2-DE gels of patients with PLCH and it was significantly over-expressed in smokers than no-smoker controls. In conclusion antioxidant defense could be involved in PLCH pathogenesis being the expression of antioxidant proteins significantly different in BAL of patients with respect to smoker or no smoker controls.