RT Journal Article
SR Electronic
T1 Quantitative methylation profiles of multiple genes in patients with non-small cell lung cancer and its association with clinicopathological correlations
JF European Respiratory Journal
JO Eur Respir J
FD European Respiratory Society
SP p1952
VO 38
IS Suppl 55
A1 Kontic, Milica
A1 Stojsic, Jelena
A1 Jovanovic, Dragana
A1 Bunjevacki, Vera
A1 Ognjanovic, Simona
A1 Puumala, Susan
A1 Nelson, Heather
YR 2011
UL http://erj.ersjournals.com/content/38/Suppl_55/p1952.abstract
AB Introduction: Aim of this study was to examine quantitative methylation patterns using pyrosequencing in multiple genes in tumor, matching normal lung tissue and blood of patients with non-small cell lung cancer. We also analyzed possible associations between clinicopathologic features of patients and DNA methylation.Material and methods: Primary tumor samples (n=65), corresponding nonmalignant lung tissues (n=65) and blood samples (n=51) were obtained from NSCLC patients, treated with curative resectional surgery. Hypermethylation status was quantified at multiple CpG sites within each promoter in multiple genes- SOX1, RASSF1A, HOXA9, CDH13, MGMT, ESR1 and DAPK by pyrosequencing.Results: For most of the genes there was a significant difference between tumor methylation, normal tissue and blood samples. Methylation in tumors was significantly higher then in normal lung for SOX1, DAPK, RASSF1A, HOXA9 and CDH13. Tumor hypermethylation was more frequently for adenocarcinoma at CDH13 and ESR1. A higher proportion of SSC tumors were hypermethylated at HOXA9 and SOX1 compared to other types of NSCLC. Patients with stage four more likely had hypermethylation at MGMT and patients with hypermethylation at HOX9 more likely had stage two and three tumors. Gender was associated with hypermethylation at CDH13 with females being more likely to have hypermethylated tumors.Conclusion: Our results show that elevated methylation levels observed in genes SOX1, RASSF1A, HOXA9, CDH13, MGMT, ESR1 and DAPK were cancer-specific and associated with some clinicopathologic features of patients.