RT Journal Article
SR Electronic
T1 Increased expression of IL-19 in metaplastic epithelium of patients with chronic rhinosinusitis and nasal polyps
JF European Respiratory Journal
JO Eur Respir J
FD European Respiratory Society
SP p3085
VO 38
IS Suppl 55
A1 Elisabetta Pace
A1 Valeria Scafidi
A1 Danilo Di Bona
A1 Liboria Siena
A1 Giuseppina Chiappara
A1 Maria Ferraro
A1 Stefania La Grutta
A1 Salvatore Gallina
A1 Riccardo Speciale
A1 Antonella Ballacchino
A1 Claus Bachert
A1 Jean Bousquet
A1 Mark Gjomarkaj
YR 2011
UL http://erj.ersjournals.com/content/38/Suppl_55/p3085.abstract
AB Chronic rhinosinusitis (CRS) (including nasal polyps) is an inflammation of the nose and of the paranasal sinuses. Nasal polyposis (NP) is common in many patients with severe asthma. The pathogenesis of NP is poorly understood.The aim of the present study was to identify biomarkers for NP. We collected nasal biopsies from normal subjects without CRS (n=12), from subjects with CRS but without NP (n=10) and from patients with CRS and NP (n=10). We used Human Asthma Gene Array and real time PCR to evaluate gene expression and western blot analysis and immunohistochemistry for protein expression.Human Asthma Gene Array showed an evident increase in IL-19 gene expression in NP from patients with CRS and NP in comparison to mucosa from inferior turbinate of normal subjects. Real time PCR confirmed the IL-19 mRNA up-regulation in patients with CRS and NP and showed an up-regulation of IL-19, at lower extent, also in the mucosa from inferior turbinate of patients with CRS in comparison to normal subjects. Western blot analysis confirmed that IL-19 is increased also at protein level in patients with CRS and NP in comparison to normal subjects. Immunohistochemistry showed that in NP IL-19 is highly expressed in the metaplastic nasal epithelium (score 4-9) when compared to normal or hyperplastic epithelium (score 1-3). When patients with CRS and NP were classified on the basis of the presence or of the absence of allergy, no differences were observed in terms of IL-19 expression.In conclusion, the results of the present study provide compelling evidence on the putative use of IL-19 as remodelling biomarker for identifying patients with chronic rhinosinusitis and NP.