RT Journal Article
SR Electronic
T1 Reduces inflammatory parameters in airways of diabetic-antigen sensitized guinea pigs
JF European Respiratory Journal
JO Eur Respir J
FD European Respiratory Society
SP p1789
VO 38
IS Suppl 55
A1 Kolahian, Saeed
A1 Asadi, Farzad
A1 Nassiri, Seyed Mahdi
YR 2011
UL http://erj.ersjournals.com/content/38/Suppl_55/p1789.abstract
AB It is well established that there is a negative association between asthma and type I diabetes and relative lack of insulin in an organism results in an overall reduction in inflammatory reactions. This study was planned to determine the inflammatory events in antigen sensitized diabetic guinea pigs. Twenty-five male guinea pigs were categorized into five groups of five each as follows: diabetic, antigen sensitized, diabetic-antigen sensitized, insulin-treated diabetic-antigen sensitized and control animals. Induction of experimental diabetes and antigen sensitization were performed by injection of streptozotocin and ovalbumine, respectively. Animals were killed by exsanguination and bronchoalveolar lavage (BAL) was performed. BAL fluid cellular and protein contents were determined. Airway responsiveness to acetylcholine was assessed. Histopathological examinations were performed on the lungs. Decreases in the airway reactivity in diabetic-antigen sensitized animals were found compared to antigen sensitized animals. Experimental diabetes decreased antigen-induced protein leakage into the airspace as well as the accumulation of inflammatory cells in BAL fluid of antigen sensitized animals. Histopathological results showed that coinduction of experimental diabetes significantly reduced the number of eosinophils in the lungs of antigen sensitized animals. Treatment with insulin completely reversed all mentioned results in the antigen sensitized diabetic animals. Experimental diabetes causes were found to decrease the airway reactivity and inflammatory responsiveness induced by antigen sensitization due to a reduction in the insulin levels.