@article {Dongp4828, author = {Wenfang Dong and Xiaojian Zhou and Jianguo Hong}, title = {Effect of leukotriene receptor antagonist on expression and variation of TGF-beta in T lymphocyte for mild persistent asthma in children}, volume = {38}, number = {Suppl 55}, elocation-id = {p4828}, year = {2011}, publisher = {European Respiratory Society}, abstract = {Objective: To investigate the role of TGF-β1 and action of montelukast (MT) in the pathogenesis of asthma in children.Methods: A single-blind, placebo-controlled trail was conducted in 60 mild persistent asthma children (5-14 y). Patients were randomly assigned to receive 5mg MT or placebo for 12 weeks. 30 healthy children (5-14 y) were as control. Clinical and pulmonary function were assessed before and after treatment. The mRNA expression of TGF-β1 in PBMC cells, subtypes of FoxP3+CD4+ T cells and TGF-β1 in plasma were measured.Result: After treatment, symptomes were improved with signigicant increase in pulmonary function in asthmatic, MT superior to placebo (p\<0.05). The mRNA expression of TGF-β1 and TGF-β1 level in asthmatic were lower than that in control (0.312{\textpm}0.07 vs 0.607{\textpm}0.2, p\<0.05 and 0.445{\textpm}0.13 vs 0.32{\textpm}0.04, p\<0.05, respectively). Total FoxP3+CD4+ cell and CD45RA+Foxp3lo were higher in asthmatic (8.3\%{\textpm}1.3\% vs 6.05\%{\textpm}1.8\%, p=0.007 and 4.60\%{\textpm}1.04\% vs 3.27\%{\textpm}1.03\%, p=0.011, respectively). CD45RA-Foxp3hi was lower (0.75\%{\textpm}0.13\% vs 0.93\%{\textpm}0.26\%, p=0.021). After treatment, CD45RA-Foxp3hi was increased in MT compared to placebo group (1.16\%{\textpm}0.24\% vs 0.89\%{\textpm}0.22\%, p=0.01). TGF-β1 levels had no correlation with those levels of pulmonary function.Conclusion: The expression of TGF-β1 was low in asthmatic children. Insufficient secretion of TGF-β1 and the defection in activating FoxP3+CD4+ Treg cells might play an important role in pathogenesis of asthma. Up-regulation of expression of TGF-β1 and induction of expression of CD45RA-Foxp3hi in Foxp3+CD4+Treg cells by MT may be one of the mechanisms by which airway inflammation is inhibited in asthma.}, issn = {0903-1936}, URL = {https://erj.ersjournals.com/content/38/Suppl_55/p4828}, eprint = {https://erj.ersjournals.com/content}, journal = {European Respiratory Journal} }