TY - JOUR T1 - Monitoring PGD<sub>2</sub> production in airway disease JF - European Respiratory Journal JO - Eur Respir J VL - 38 IS - Suppl 55 SP - p3348 AU - Johan Larsson AU - David Balgoma AU - Ingrid Delin AU - Theo Gülen AU - Gunnar Nilsson AU - Hans Hägglund AU - Kameran Daham AU - John Lawson AU - Garret FitzGerald AU - Barbro Dahlén AU - Craig Wheelock AU - Sven-Erik Dahlén Y1 - 2011/09/01 UR - http://erj.ersjournals.com/content/38/Suppl_55/p3348.abstract N2 - Introduction: Prostaglandin (PG) D2 is a major mast cell mediator implicated in asthma and airway disease. Measurement of the urinary levels of 9α,11β-PGF2 has been established to monitor PGD2 production. Tetranor prostaglandin D (PGDM) is however the major urinary metabolite of PGD2.Aims and objectives: To evaluate the use of urinary levels of 9α,11β-PGF2 vs. PGDM. In addition, the use of enzyme immune assay (EIA) was compared with LC-MS/MS.Methods: Urinary samples from four studies were used; bronchoprovocation using allergen (n=9) and aspirin in aspirin-intolerant asthma (n=11), effect of celecoxib on baseline excretion (n=19), and excretion in patients with mastocytosis or anaphylaxis (M/A) during exacerbation (n=15). Urinary excretion of 9α,11β-PGF2 and PGDM were measured by specific EIAs (Cayman Chemical) and LC-MS/MS, and data expressed as ng/mmol creatinine.Results: The levels of PGDM (mean±SEM) before allergen challenge were 1330±348 and 1236±192 after (p&gt;0.05), the corresponding values of 9α,11β-PGF2 were 40±4 and 77±10 (p=0.002), respectively.With aspirin challenge levels of PGDM were 516±82 before and 609±95 after (p&gt;0.05), whereas the levels of 9α,11β-PGF2 were 56±16 and 75±24 (p=0.002), respectively.In M/A the levels of PGDM and 9α,11β-PGF2 were 1977±886 (p&lt;0.05 for PGDM vs baseline in healthy subjects) and 251±66 respectively, with values mildly correlated (r=0.698, p=0.00382).Bland-Altman analysis showed good agreement between PGDM measurements with MS and EIA.Conclusion: The earlier metabolite 9α,11β-PGF2 may be more suitable to follow the dynamics of release during challenges whereas the higher levels of PGDM may be more appropriate for monitoring global differences in baseline production of PGD2. ER -