TY - JOUR T1 - Non-invasive measurement of respiratory impedance in conscious guinea pigs utilising impulse oscillometry JF - European Respiratory Journal JO - Eur Respir J VL - 38 IS - Suppl 55 SP - p1191 AU - Carmen Claudia Klein AU - Jens Thomas AU - Norbert Mieskes AU - Martin Hoffmann AU - Hans-Juergen Smith Y1 - 2011/09/01 UR - http://erj.ersjournals.com/content/38/Suppl_55/p1191.abstract N2 - Aim: Since rodents are often utilised as models in respiratory research, we investigated a newly constructed Impulse Oscillometry System (IOS) which was modified to allow non invasive measurements of respiratory impedance in conscious guinea pigs.Material and methods: Five female guinea pigs (body weight: 592.2±73.0 g) were included in the study. The animals were fixated manually in an extended supine position. a) Three consecutive IOS measurements, taken within a few minutes duration from each animal were used to evaluate short time variability. b) The results of measurements taken on three consecutive days at the same time of the day were used to assess long time variability. Coefficients of variation (CV%) were calculated for oscillometric resistance parameters between 5 and 35 Hz.Results: Coefficients of variation (CV%) for Resistance parameters at selected frequencies are shown in table 1.View this table:Table 1. Coefficients of variation (in %) for spectral Resistance in a short-time (study A) and a long-time study (study B) in five conscious guinea pigs; means and standard deviationsConclusion: Putting awake guinea pigs in supine body position they get into a sleep-like condition. This was the preferred procedure to get well reproducible results of impedance spectra utilising impulse oscillometry in this species.The authors appreciate the financial support of the German Federal Ministry of Economics (Berlin Germany, registration number IW 070139). ER -