TY - JOUR T1 - Gene regulation of apoptosis and telomere length in COPD and lung cancer JF - European Respiratory Journal JO - Eur Respir J VL - 38 IS - Suppl 55 SP - p707 AU - Dimitry Bazyka AU - Iryna Ilyenko AU - Lyudmila Shvajko AU - Kostyantyn Bazyka AU - Oleg Belyaev Y1 - 2011/09/01 UR - http://erj.ersjournals.com/content/38/Suppl_55/p707.abstract N2 - The aim of this study was to investigate the role of gene regulaton of apoptosis (TP53, TP53 I3, FASLG, BAX, CCND1) and telomere function (TRF1, TRF2) in peripheral blood lymphocytes in COPD and lung cancer.Methods: Groups included 16 COPD patients (FEV1 41-64), 5 lung cancer and 12 controls. Relative telomere length and apoptosis in peripheral blood cells were analyzed by flow cytometry. Relative quantification (RQ) of gene expression was investigated by the RT-PCR using 7900 HT System and TaqMan technology.Results: In COPD a decreased RQ was shown for FASLG, TP53 and TP53 I3 genes with overexpression of BAX (p<0,001) and CCND1 genes and increased presentation of BAX-protein and MAPK14 (p38) on CD16+56+ cells by flow cytometry.A significant decrease was demonstrated TERF1 and especially TERF2 expression (Fig. 1). In lung cancer high Ki-67+ and HER2+ cell counts were combined with a decrease of BIRC5 and FASLG expression and MADD, MAPK14 and CDKN1B over-expression. The RTL was higher in lung cancer as comparing with COPD and control but no correlation was seen with TERT or TERF2 gene expression.Conclusion: In COPD the association is demonstrated between the cytotoxic cells activation, MAPK and CCND1 genes and decreased apoptosis. Some independence is shown between TERT and T-loop genes expression and the telomere length in lung cancer and COPD, supposing the influence of different regulation pathways. ER -