RT Journal Article
SR Electronic
T1 Evaluation of the <em>Legionella</em> <em>V</em>-TesT compared to the BinaxNOW to detect <em>Legionella</em> serogroup 1 antigen in urine samples
JF European Respiratory Journal
JO Eur Respir J
FD European Respiratory Society
SP p2464
VO 38
IS Suppl 55
A1 Evy De Witte
A1 Katherine Loens
A1 Christine Lammens
A1 Jordy Almirall
A1 Herman Goossens
A1 Greet Ieven
YR 2011
UL http://erj.ersjournals.com/content/38/Suppl_55/p2464.abstract
AB The performance and user friendliness of a new immunochromatographic assay, the Legionella V-tesT (Coris Bioconcept) for the detection of Legionella pneumophila serogroup 1 antigen in urine (uAg) were evaluated by comparing its results with the BinaxNOW Legionella uAg-test results (Inverness Medical).For direct uAg testing using both tests, 129 previously collected and frozen urine samples were used: 61 specimens from pneumonia patients during a Legionella outbreak: 34 from the Hospital of Matarό (Spain), 27 from the outbreak in Kapellen (Belgium, 1999) and 68 urine samples from patients with lower respiratory tract infections other than Legionella infections enrolled in the European GRACE study. In case of discrepant results, urine samples were tested with the Biotest. Results of culture and/or PCR on respiratory samples were included to conclude on the true positive status of the patient. The number of manipulations and the turn around time (TAT) were evaluated as well.41/129 (32%) samples tested were BinaxNOWpositive. The sensitivity and specificity of the V-TesT in comparison to the BinaxNOW were 97.6% and 97.7% respectively.View this table:Results for the evaluation of the V-tesT in comparison to the BinaxNOW LegionellaResults yielded 2 apparent false positives and 1 false negative: all were confirmed as true positive Legionella infections. The TAT was comparable but the new V-TesT could be used directly on the urine specimen, while the BinaxNOW required two additional steps.The V-TesT has comparable performance characteristics to the BinaxNOW Legionella and is an even more user-friendly test.