TY - JOUR T1 - LSC 2011 Abstract: Cigarette smoke-induced inflammation promotes melanoma cell metastasis in lung parenchyma JF - European Respiratory Journal JO - Eur Respir J VL - 38 IS - Suppl 55 SP - p1478 AU - Sandrine Bekaert AU - Natacha Rocks AU - Maud Gueders AU - Geneviève Paulissen AU - Agnès Noël AU - Didier Cataldo Y1 - 2011/09/01 UR - http://erj.ersjournals.com/content/38/Suppl_55/p1478.abstract N2 - It is only during the last decade that clear evidence has been obtained that inflammation plays a critical role in different stages of tumor development, including initiation, promotion, malignant conversion, invasion and metastasis. There is increasing evidence that an inflammatory microenvironment is an essential component of all tumors (Psaila and Lyden, Nat Rev Cancer, 2009 Apr;9(4):285-93).In the present study, we assessed in vivo the impact of cigarette smoke (CS) on the tumor cell extravasation in lungs after tail vein injection of B16F10 melanoma cells. We first characterized airway inflammation obtained after smoke exposure (reference cigarettes 3R4F) for varied time periods (1, 2, 4, 8 and 12 weeks). Smoke exposure was performed 5 days a week. Neutrophils, alveolar macrophages, interstitial macrophages, dendritic cells, T cells and natural killer T (NKT) cells, were characterized in lung tissues of mice exposed to CS and AIR using flow cytometry. In Vitro, the direct effect of cigarette smoke extract (CSE) on proliferation of B16F10 melanoma cells was determined for 1 to 5 days. In vivo, mice exposed for 2 weeks to cigarette smoke or air were injected with B16F10 melanoma cells in the tail vein. After 3 weeks, hematoxylin-eosin staining allowed quantification of lung metastasis (tumor area/total lung area). An increase of metastasis and implantation site in lungs was observed in CS exposed group. Conceivably, CS constituents significantly promote extravasation of melanoma cells in lung tissues. The mechanism or signaling pathway responsible for this dissemination needs to be further investigated. ER -