PT  - JOURNAL ARTICLE
AU  - Marcin Skrzypski
AU  - Rafal Dziadziuszko
AU  - Amelia Szymanowska
AU  - Ewa Jassem
AU  - Piotr Czapiewski
AU  - Wojciech Biernat
AU  - Witold Rzyman
AU  - Ryszard Pawlowski
AU  - Jacek Jassem
TI  - The risk of distant metastases and prognosis prediction in early stage squamous cell lung cancer (SqCLC) by means of 3 microRNAs expression assessment
DP  - 2011 Sep 01
TA  - European Respiratory Journal
PG  - p1953
VI  - 38
IP  - Suppl 55
4099  - http://erj.ersjournals.com/content/38/Suppl_55/p1953.short
4100  - http://erj.ersjournals.com/content/38/Suppl_55/p1953.full
SO  - Eur Respir J2011 Sep 01; 38
AB  - Background: About 50% of NSCLC patients (pts) develop distant metastases following surgery. Currently, apart from stage there are no factors to select high risk pts for adjuvant chemotherapy. We previously showed prognostic value of selected microRNAs (miRs) in frozen tissues of SqCLC and the feasibility of their expression assessment in formalin fixed paraffin embedded (FFPE) samples (Skrzypski et. al. JCO 28;521s:2010). In this study we validated the prognostic value of the 3-miR signature assessed in the FFPE samples in an independent patient population.Methods: FFPE tumor tissue was obtained from 89 stage I-II SqCLC pts. Of those, 40 pts developed distant metastases and 49 had no relapse after a median follow-up of 5.4 years (range, 3.4-8.2 years). Expression of miR-10b, miR-532-3p and miR-192* was analyzed by RT-PCR assays and normalized vs. U6 expression and calibrated by ΔΔCt method. After z-score transformation, the risk score was calculated based on the expression of these 3 miRs.Results: The median metastasis-free survival (MFS) was not reached in the low risk group according to 3-miR expression signature, whereas it was 26 months in the high risk group. The 3-miR (10b, 532-3p and 192*) expression signature was significantly related to the MFS (log-rank; p=0.013). With the median of the risk score as a cut-off, the sensitivity for distant relapse prediction was 66% at the specificity of 64%. After exclusion of stage IA pts, the corresponding values were 73% and 69%.Conclusions: 3-miR expression profile has been successfully validated as a predictor of dissemination in operable early stage squamous cell lung cancer.