@article {Abidip677, author = {Anouar Abidi and Moncef Feki and Sloua Ben Khamsa}, title = {Effect of experimental pulmonary fibrosis induced by bleomycin on the lung fatty acid composition in Wistar rats}, volume = {38}, number = {Suppl 55}, elocation-id = {p677}, year = {2011}, publisher = {European Respiratory Society}, abstract = {Background: Many recent studies are directed towards the use of natural substances for the treatment of pulmonary fibrosis and of these fatty acids.The main our study was first to verify the normal composition of fatty acids in the lungs and collect the effect of experimental fibrosis induced by bleomycin in rats on the latter.Methods: A total of twenty males wistar rats which received water and food ad libitum, were divided randomly into two groups: untreated group (G1, n=10) and treated group; (G2, n=10).Pulmonary fibrosis was induced in all treated rats (G2) by bleomycin (4 mg/kg, single dose) administered intratracheally.Three day later, all rats were sacrificed, lungs were extracted and blood was collected for analysis of fatty acid composition in the lungs and red cells of rats studied, and this by using the technique of gas chromatography (GC).Results: The statistical of independent-samples T test revealed that bleomycin alters significantly the composition means of various fatty acids in treated group (G2) compared to untreated group (G1) as shown in the figures below.Figure 1. Fatty acids compostion in lungs of treated group (G2) and control group (G1).Figure 2. Fatty acids compostion in red cells of treated group (G2) and control group (G1).Conclusion: These results confirm that bleomycin affects the fatty acid composition of lung giving the opportunity to test the effects of substances rich in fatty acids on pulmonary fibrosis models in rats.}, issn = {0903-1936}, URL = {https://erj.ersjournals.com/content/38/Suppl_55/p677}, eprint = {https://erj.ersjournals.com/content}, journal = {European Respiratory Journal} }