RT Journal Article
SR Electronic
T1 Late-breaking abstract: Comparison of bone marrow derived-mononuclear cells with mesenchymal stem cells on inflammatory and remodeling processes in experimental chronic allergic asthma
JF European Respiratory Journal
JO Eur Respir J
FD European Respiratory Society
SP 3407
VO 38
IS Suppl 55
A1 Soraia Abreu
A1 Mariana Antunes
A1 Debora Xisto
A1 Milena Vasconcellos
A1 Julia Crossetti
A1 Vera Capelozzi
A1 Marcelo Morales
A1 Patricia Rocco
YR 2011
UL http://erj.ersjournals.com/content/38/Suppl_55/3407.abstract
AB Recently, we demonstrated the beneficial effects of bone marrow-derived mononuclear cells (BMDMCs) on lung morphofunction in experimental chronic allergic asthma. Mesenchymal stem cells (MSCs) have the potential to serve as a universal source for replacement of specific cells in several diseases and thus may offer hope as a potential therapeutic intervention for the treatment of the chronic remodeling changes that occur in asthma. This study aimed to compare the therapeutic effects of BMDMCs with MSCs on inflammatory and remodeling processes in experimental chronic allergic asthma. Thirty-six C57BL/6 mice were assigned to two groups. In the OVA group, mice were sensitized and repeatedly challenged with ovalbumin. Control mice (C) received saline under the same protocol. C and OVA groups were further randomized to receive saline (SAL), BMDMCs or MSCs (1×105) intratracheally 24 h after the last challenge. After one week, airway resistance, viscoelastic pressure, static elastance, as well as the degree of lung inflammation and remodeling (light microscopy and immunohistochemistry) were analyzed. BMDMC and MSC therapies led to a reduction in eosinophil infiltration and fibrosis in airway and lung parenchyma compared to OVA leading to a reduction in airway resistance and viscoelastic pressure. These parameters were more reduced after BMDMCs compared to MSCs. In conclusion, in the present model of chronic allergic asthma, both BMDMC and MSC therapies were effective at modulating the inflammatory and fibrogenic processes, however, BMDMC administration led to greater beneficial effect.Supported by: PRONEX, CNPq, FAPERJ, CAPES.