RT Journal Article
SR Electronic
T1 Curette lavage fluid analysis of <em>EGFR</em>, <em>KRAS</em>, and <em>P53</em> mutations in lung cancer patients
JF European Respiratory Journal
JO Eur Respir J
FD European Respiratory Society
SP p1976
VO 38
IS Suppl 55
A1 Fumihiro Yamaguchi
A1 Kunihiko Fukuchi
A1 Sakiko Tazawa
A1 Hidethugu Tateno
A1 Eisuke Kato
A1 Aya Wakabayashi
A1 Asami Tada
A1 Takuya Iwasaki
A1 Makoto Hayashi
A1 Yutaka Thuchiya
A1 Jun Yamashita
A1 Norikazu Takeda
A1 Shogo Tomita
A1 Fumio Kokubu
YR 2011
UL http://erj.ersjournals.com/content/38/Suppl_55/p1976.abstract
AB Purpose: Mutation analyses of individual lung cancer patients should provide useful information for determining treatment. When sufficient tissue samples for pathology cannot be collected by bronchoscopy, cytology is substituted to establish diagnosis. In this study, epidermal growth factor receptor (EGFR), KRAS, and P53 mutations in cells attached to the curette were analyzed by collecting the lavage fluid.Subjects: Samples were obtained from 63 lung cancer patients receiving treatment from April, 2009 to October 2010 at the Department of Respiratory Medicine, Showa University Fujigaoka Hospital. Official approval for the study was obtained in advance from the Ethics Committee for Genomic Research at Showa University.Methods: DNAs were extracted from cells in the curette lavage fluid. PCRs were performed to amplify mutation hot spot regions in EGFR, KRAS, and P53. The PCR products were direct-sequenced, and the mutations confirmed by sequencing with both forward and reverse primers.Results and discussion: Seven patients were found with EGFR mutations and seven with P53 mutations. No mutation in KRAS was identified. The mutation rates observed here for the three genes were lower than those reported previously by others. However, EGFR exon 19 deletions were identified in 6 of 53 non-small cell lung cancer patients in this study (11.3%), making it comparable to that reported elsewhere. It is felt that in instances where the number of cancer cells in the test sample is low, it becomes difficult to detect point mutations and insertions. It should be cautioned that in instances where direct sequencing is utilized, mutation detection standards may vary according to the individual facility.