TY - JOUR T1 - Analysis of EGFR-mutations in a diverse urban patient population JF - European Respiratory Journal JO - Eur Respir J VL - 38 IS - Suppl 55 SP - p2738 AU - Amanda Tufman AU - Kai Wagner AU - Andreas Jung AU - Rudolf Maria Huber Y1 - 2011/09/01 UR - http://erj.ersjournals.com/content/38/Suppl_55/p2738.abstract N2 - Background: EGFR-mutations are predictive for EGFR-TKIs in advanced NSCLC; however, not all pts are screened for these mutations. Clinical criteria (female, never-smoker, East-Asian, adeno-ca) are often used to select pts, however the impact of country of origin in non-East Asians has not yet been described. We analysed EGFR-mutation and country of origin in NSCLC pts treated at a university hospital in Munich.Methods: We retrospectively identified all pts treated for primary thoracic malignancy on the ward in 3 months and conducted a chart review. Specimens were analysed for activating mutations in exons 18, 19, 21, using nested PCR together with tailed primers (bidirectional didesoxi-sequencing applying the tail-sequences as sequencing primers on a Genetic Analyzer 3130 platform - Applied Biosystems).Results: From Oct to Dec 2009, 62 pts with thoracic malignancies (NSCLC 52, SCLC 7, carcinoid 1, mesothelioma 2) were treated. Country of origin: Ethiopia 1, Mongolia 1, Italy 2, Spain 1, Russia 1, Croatia 4 (6.5%), Turkey 9 (14.5%), Germany 43 (69.4%). Subtypes of NSCLC: adeno 48.1, large cell 3.8, squamous 40.4, NOS 7.7%. 53 samples are analyzed, and revealed 4 activating mutations and one pt with silent mutations in exon 18 and 20. Activating mutations were found in 3/43 (7%) Germans (including a heavy smoker with squamous cell ca, 1 adeno, 1 large cell), 1/9 (11%) Turks and 2 silent mutations were found in 1/4 Croatians (adeno).Discussion: Regional mutational heterogeneity occurs in various illnesses. In NSCLC this affects the generalisability of study data. In this first analysis we describe the diverse nature of a patient population in Munich, and show similar rates of activating EGFR mutation in all subgroups. ER -