TY - JOUR T1 - Lymphocytes populations in bloods and lungs in patients with auto-immune pulmonary alveolar proteinosis JF - European Respiratory Journal JO - Eur Respir J VL - 38 IS - Suppl 55 SP - p631 AU - Yoshikazu Inoue AU - Toru Arai AU - Junji Otsuka AU - Chikatoshi Sugimoto AU - Masaki Hirose AU - Akiko Natsumuro AU - Yoko Iwaki AU - Mikiko Nakagawa AU - Seiji Hayashi AU - Masaji Okada Y1 - 2011/09/01 UR - http://erj.ersjournals.com/content/38/Suppl_55/p631.abstract N2 - Background and aim: Pulmonary alveolar proteinosis (PAP) is a rare lung disease which is characterized by pulmonary surfactant accumulation in the lungs, and a subset of PAP which occurs in association with granulocyte/macrophage colony-stimulating factor (GM-CSF) autoantibodies is recommended to call autoimmune pulmonary alveolar proteinosis (APAP) (Inoue Y et al. Am J Respir Crit Care Med. 2008 177:752). We hypothesize that cellular immunity contributed to pathogenesis of APAP. The aim of this study is to know the lymphocytes populations and to clarify the role of lymphocytes in APAP.Subjects and methods: Peripheral bloods (PBMC) and bronchoalveolar lavage fluids (BAL) from 30 patients with APAP, 20 healthy volunteers (HV), and 15 patients with other control diseases (CDs: IIPs, sarcoidosis, etc.) were analyzed. CD3, CD4, CD8, CD19, CD20, CD69, CD3/19, CD3/16, CD3/56, CD16/56, CD4/25, CD14/16, and CD19/69 were measured by flow cytometry.Results: CD3+T cells in PBMC were lower in APAP than HV. CD3+/CD16- in PBMC were significantly lower in APAP than HV. CD4 in PBMC positively correlated with%VC, and negatively correlated with A-aDO2. CD3+T cells in BAL were significantly lower than CDs.Conclusion: We found abnormal T cell populations in APAP, which suggests possible association of T cell immunity on the pathogenesis of APAP.This study was partially supported by the grant-in-aid for scientific research, the grant from Japanese Ministry of Health, Labour, and Welfare, and the grant from National Hospital Organization. ER -