RT Journal Article
SR Electronic
T1 Expression patterns of IL-1 receptor 1 in COPD lungs
JF European Respiratory Journal
JO Eur Respir J
FD European Respiratory Society
SP p3822
VO 38
IS Suppl 55
A1 Anders Bergqvist
A1 Cecilia Andersson
A1 Michiko Mori
A1 Chris Van Hove
A1 Leif Bjermer
A1 Jonas Erjefält
YR 2011
UL http://erj.ersjournals.com/content/38/Suppl_55/p3822.abstract
AB Rationale: Recent research in murine models suggests an important role of Interleukin-1 receptor 1 (IL-1R1) signalling in the pathogenesis of COPD. Yet, the features of IL-1R1 expression in human COPD patients remain poorly investigated. This study characterizes the expression of IL-1R1 in lung tissues from COPD patients and control subjects.Methods: Lung resections were obtained from 27 COPD patients; GOLD I (n=6), GOLD II-III (n=13) and GOLD IV (n=8). Never smoking controls (n=7) and smoking controls (n=6) served as controls. IL-1R1 expression was studied by immunohistochemistry and computerized image analysis. A double staining immunofluorescence protocol visualized IL-1R1 expressing cell populations.Results: The expression of IL-1R1 in small airway epithelium was significantly higher in GOLD IV vs. GOLD II-III patients (p=0.03). In the small airway lamina propria, there was no overall statistical difference between the groups (p=0.12), but there was a tendency towards a higher IL-1R1 expression in GOLD IV vs. GOLD II-III patients (uncorrected p=0.02). In the endothelium, IL-1R1 expression was similar between the groups (overall p=0.8). IL-1R1 was found to be expressed on multiple leukocyte populations such as T-cells, dendritic cells, macrophages and NK-cells.Conclusions: Our data suggest that at baseline conditions IL-1R1 is expressed in the airway epithelium, endothelial cells, and on multiple subepithelial leukocyte populations. Severe stages of COPD appear to be associated with increased IL-1R1 expression in small airway epithelium and in infiltrating cells in the lamina propria, a phenomenon that may participate in the abnormal immune reactions in advanced COPD.