PT  - JOURNAL ARTICLE
AU  - Evgeny Babarskov
AU  - Evgeny Stepanov
AU  - Yury Shulagin
AU  - Alexander Cherniak
AU  - Zaurbek Aisanov
AU  - Alexander Chuchalin
TI  - Non-invasive method for investigation of inhaled carbon monoxide (CO) distribution between intra- and extravascular compartments
DP  - 2011 Sep 01
TA  - European Respiratory Journal
PG  - p1202
VI  - 38
IP  - Suppl 55
4099  - http://erj.ersjournals.com/content/38/Suppl_55/p1202.short
4100  - http://erj.ersjournals.com/content/38/Suppl_55/p1202.full
SO  - Eur Respir J2011 Sep 01; 38
AB  - Background: Therapeutic potential of inhaled CO at low concentration was demonstrated in the treatment of some human diseases [Ryter S. at al. Am J Respir Cell Mol Biol 2009; 41: 251-260]. Basic parameters of CO-dosing were: CO concentration in inhaled air, time of administration and carboxyhemoglobin (COHb) concentration in blood. However therapeutic effect of CO is defined mainly by interaction with intracellular enzymes in extravascular tissues.Aim: To develop method for determination of CO contents in extravascular compartment.Methods: Balance equation of total CO mass administrated into body (MCOt, mkmol) used for calculation of CO contents in extravascular compartment (MCOex, mkmol). COHb concentration (Ccohb,%) was defined by measuring of equilibrium CO concentration (Ceq, ppm) in exhaled air using high selective laser spectrophotometer. Total Hb mass (MHb,g) detected by preliminary administration of calculated CO amount (ΔMco, mkmol) as a result of single breath diffusion capacity test (Master Lab, Erich Jaeger) in 19 healthy volunteers (12 males and 7 females).Results: ΔMco (a part of MCOt) varied from about 100 to 600 mkmol and Ceq deviations from baseline values (ΔCeq) were 2/8ppm. Following formula was found: MHb=8.95*ΔMco/ΔCeq. MHb was approximatly proportional to body weight (Mbody, kg). The ratio R=MHb/Mbody was equal about 9.8 for males and 8.0 for females. After additonal CO adminstration intravascular CO contents (MCOin, mkmol) is defined by formula: MCOin=0.11*ΔCeq*MHb, consequently MCOex=MCOt-MCOin.Conclusion:Investigation of administrated CO distribution between compartments allow to optimize dosing regime and total CO dose value.