RT Journal Article
SR Electronic
T1 Effect of thrombin inhalation in a murine model of bronchial asthma
JF European Respiratory Journal
JO Eur Respir J
FD European Respiratory Society
SP p4104
VO 38
IS Suppl 55
A1 Takehiro Takagi
A1 Masahiro Onishi
A1 Masahiro Naito
A1 Atsushi Fujiwara
A1 Tetsu Kobayashi
A1 Hiroyasu Kobayashi
A1 Corina D'Alessandro-Gabazza
A1 Esteban Gabazza
A1 Osamu Taguchi
YR 2011
UL http://erj.ersjournals.com/content/38/Suppl_55/p4104.abstract
AB Background: Thrombin is the effector enzyme of the coagulation system with important biological functions not only in thrombosis and hemostasis but also in inflammation. The precise role of thrombin in allergy remains unknown but recently there are some reports showing that thrombin plays an important role in the pathogenesis of asthma. In addition, previously we reported that thrombin inhibitor did not improve allergic response in murine asthma model.Objectives: To evaluate the effect of inhaled thrombin on airway inflammation and hyperresponsiveness in a murine asthma model.Methods: Bronchial asthma was induced by sensitization and challenge with ovalbumin (OVA). Littermates treated with saline were used as controls. The effect of inhaled different dose of thrombin was assessed by administering prior to OVA exposure. Airway inflammation was evaluated by measuring the number of inflammatory cells and the level of cytokine in bronchoalveolar lavage fluid (BAL). Airway hyperresponsiveness was measured using a plethysmograph.Results: The levels of IgE, IL-5, and the number of eosinophils in BAL were decreased in low dose of thrombin treated mice compared to saline treated mice. The degree of airway hyperresponsiveness was significantly decreased in low dose of thrombin treated mice as compared to saline treated mice.Conclusion: These results suggest thrombin exerts a differential effect in bronchial asthma depending on its concentration.