RT Journal Article
SR Electronic
T1 Effect of oxidative stress on respiratory epithelium from children with Down syndrome
JF European Respiratory Journal
JO Eur Respir J
FD European Respiratory Society
SP 1037
OP 1045
DO 10.1183/09031936.00122812
VO 42
IS 4
A1 Martijn Bruijn
A1 René Lutter
A1 Eric Eldering
A1 Albert P. Bos
A1 Job B.M. van Woensel
YR 2013
UL http://erj.ersjournals.com/content/42/4/1037.abstract
AB Children with Down syndrome are at high risk for acute respiratory distress syndrome. In Down syndrome, both regulation of inflammation and apoptosis, important in acute respiratory distress syndrome pathophysiology, are abnormal. This has been linked to an imbalance in free radical scavengers. We investigated the expression of free radical scavengers and the effect of oxidative stress in terms of apoptosis and inflammation in respiratory epithelium from children with Down syndrome compared with control subjects.We cultured primary nasal epithelial cells from Down syndrome children (n=12) and controls (n=17) and exposed them to oxidative stress by supplementing superoxide.First we showed that the expression of the free radical scavengers CuZn-superoxide dismutase was 28% higher (p=0.06), catalase was 36% lower (p=0.04) and glutathione peroxidase was 73% lower (p=0.004) in Down syndrome children compared with controls. We found no significant difference in apoptosis, between Down syndrome and control subjects after exposure to oxidative stress. We also found no significant difference in levels of interleukin (IL)-1β, IL-6, IL-8, vascular endothelial growth factor and granulocyte colony-stimulating factor in primary nasal epithelial cell supernatant after exposure to oxidative stress between Down syndrome and control subjects.We found an imbalance in free radical scavengers in respiratory epithelial cells from children with Down syndrome, but this did not result in increased levels of either apoptosis or inflammation upon exposure to oxidative stress.Free radical scavenger imbalance: respiratory apoptosis or inflammation not increased in Down syndrome children http://ow.ly/mxypE