RT Journal Article
SR Electronic
T1 Effects of cigarette smoke and hypoxia on pulmonary circulation in the guinea pig
JF European Respiratory Journal
JO Eur Respir J
FD European Respiratory Society
SP 617
OP 627
DO 10.1183/09031936.00105110
VO 38
IS 3
A1 E. Ferrer
A1 V.I. Peinado
A1 J. Castañeda
A1 J. Prieto-Lloret
A1 E. Olea
A1 M.C. González-Martín
A1 M.V. Vega-Agapito
A1 M. Díez
A1 D. Domínguez-Fandos
A1 A. Obeso
A1 C. González
A1 J.A. Barberà
YR 2011
UL http://erj.ersjournals.com/content/38/3/617.abstract
AB Cigarette smoke (CS) and chronic hypoxia (CH) can produce pulmonary hypertension. Similarities and differences between both exposures and their interaction have not been explored. The aim of the present study was to investigate the effects of CS and CH, as single factors or in combination, on the pulmonary circulation in the guinea pig. 51 guinea pigs were exposed to CS for 12 weeks and 32 were sham-exposed. 50% of the animals in each group were additionally exposed to CH for the final 2 weeks. We measured pulmonary artery pressure (Ppa), and the weight ratio between the right ventricle (RV) and left ventricle plus the septum. Pulmonary artery contractility in response to noradrenaline (NA), endothelium-dependent vasodilatation and distensibility were evaluated in organ bath chambers. The number of small intrapulmonary vessels showing immunoreactivity to smooth muscle (SM) α-actin and double elastic laminas was assessed microscopically. CS and CH induced similar increases of Ppa and RV hypertrophy (p<0.05 for both), effects that were further enhanced when both factors were combined. CH increased the contractility to NA (p<0.01) and reduced the distensibility (p<0.05) of pulmonary arteries. Animals exposed to CS showed an increased number of small vessels with positive immunoreactivity to SM α-actin (p<0.01) and those exposed to CH a greater proportion of vessels with double elastic laminas (p<0.05). We conclude that CH amplifies the detrimental effects of CS on the pulmonary circulation by altering the mechanical properties of pulmonary arteries and enhancing the remodelling of pulmonary arterioles.