TY - JOUR T1 - Increased lung neutrophil apoptosis and inflammation resolution in nonresponding pneumonia JF - European Respiratory Journal JO - Eur Respir J SP - 1158 LP - 1164 DO - 10.1183/09031936.00190410 VL - 38 IS - 5 AU - I. Moret AU - M.J. Lorenzo AU - B. Sarria AU - E. Cases AU - E. Morcillo AU - M. Perpiñá AU - J.M. Molina AU - R. Menéndez Y1 - 2011/11/01 UR - http://erj.ersjournals.com/content/38/5/1158.abstract N2 - Neutrophil activation state and its relationship with an inflammatory environment in community-acquired pneumonia (CAP) remain insufficiently elucidated. We aimed to evaluate the neutrophil apoptosis and cytokine pattern in CAP patients after 72 h of treatment, and their impact on infection resolution. Apoptosis of blood and bronchoalveolar lavage (BAL) neutrophils was measured in nonresponding CAP (NCAP), in responding CAP (blood only) and in patients without infection (control). Pro-inflammatory (interleukin (IL)-6, IL-8) and anti-inflammatory (IL-10) cytokines were measured. Main outcomes were clinical stability and days of hospitalisation. Basal neutrophil apoptosis was higher in the BAL and blood of NCAP, whereas spontaneous apoptosis (after 24 h culture) was lower. Cytokines in NCAP were higher than in responding CAP and control: IL-6 was increased in BAL and blood, IL-8 in BAL and IL-10 in blood. An increased basal apoptosis (≥20%) in BAL of NCAP was associated with lower systemic IL-10 (p<0.01), earlier clinical stability (p=0.05) and shorter hospital stay (p=0.02). A significant correlation was found for systemic IL-6 and IL-10 with days to reach stability and length of stay. After 72 h of treatment, an increased basal alveolar neutrophil apoptosis might contribute to downregulation of inflammation and to faster clinical stability. ER -