PT  - JOURNAL ARTICLE
AU  - D Nowak
AU  - A Antczak
AU  - T Pietras
AU  - P Bialasiewicz
AU  - M Krol
TI  - Protective effect of ambroxol against heat- and hydrogen peroxide-induced damage to lung lipids in mice
AID  - 10.1183/09031936.94.07091629
DP  - 1994 Sep 01
TA  - European Respiratory Journal
PG  - 1629--1634
VI  - 7
IP  - 9
4099  - http://erj.ersjournals.com/content/7/9/1629.short
4100  - http://erj.ersjournals.com/content/7/9/1629.full
SO  - Eur Respir J1994 Sep 01; 7
AB  - We wanted to determine whether ambroxol, a drug which stimulates the release of surfactant by type II pneumocytes, can protect lung lipids from peroxidative damage in mice. Animals were injected intraperitoneally with ambroxol, 0.169 mmol.kg-1, or 1 ml buffer once a day for three consecutive days. Lipid peroxidation was then induced in lung homogenates either by means of heat, 50 degrees C, or H2O2, 10 mmol.l-1. The lung homogenates from ambroxol-treated animals revealed decreased lipid peroxidation in response to both stimuli. The heat- and H2O2-induced generation of conjugated dienes (a first lipid peroxidation product) in ambroxol-treated lung homogenates was 3.7 and 3.1 fold lower than in the lungs from buffer-injected mice. Ambroxol, as an inhibitor of heat- and H2O2-induced lipid peroxidation, was equipotent to and stronger than the two antioxidants, N-acetylcysteine and methionine, respectively. Ambroxol was not able to protect heart and liver lipids. These results suggest that ambroxol can sufficiently enhance the antioxidant defence in lung tissue and can act as a lung lipid antioxidant.