RT Journal Article
SR Electronic
T1 Effect of frusemide on bradykinin- and capsaicin-induced contraction of the guinea-pig trachea
JF European Respiratory Journal
JO Eur Respir J
FD European Respiratory Society
SP 434
OP 439
DO 10.1183/09031936.93.06030434
VO 6
IS 3
A1 Molimard, M
A1 Advenier, C
YR 1993
UL http://erj.ersjournals.com/content/6/3/434.abstract
AB Frusemide, a loop diuretic, inhibits the bronchial response to various bronchoconstrictor stimuli in asthmatic subjects. The underlying mechanisms remain unclear. In order to determine whether frusemide inhibits pharmacologically induced C-fibre stimulation, we investigated the effect of frusemide on bradykinin-, capsaicin-, neurokinin A-, and substance P-induced contraction of the guinea-pig isolated trachea. Frusemide 10(-5) and 10(-4) M produced a significant inhibition of concentration-response curves to bradykinin, which was markedly reduced by indomethacin 10(-6) M. Frusemide significantly reduced capsaicin-induced contraction only in the presence of indomethacin 10(-6) M. Neurokinin A- and substance P-induced contractions were not affected by frusemide and/or indomethacin. Our data suggest that a cyclo-oxygenase pathway is involved in the inhibition by frusemide of the bradykinin-induced contraction, but not in the inhibition of the capsaicin-induced contraction.