RT Journal Article
SR Electronic
T1 FIZZ1 potentiates the carbachol-induced tracheal smooth muscle contraction
JF European Respiratory Journal
JO Eur Respir J
FD European Respiratory Society
SP 1165
OP 1173
DO 10.1183/09031936.00097609
VO 36
IS 5
A1 H. Chen
A1 B.A. Jacobson
A1 L. Mason
A1 S.F. Wolf
A1 M.R. Bowman
YR 2010
UL http://erj.ersjournals.com/content/36/5/1165.abstract
AB FIZZ1 is an adipokine highly expressed under inflammatory conditions, and yet, little is known of its function. In this study we examine the expression and function of FIZZ1 in an ovalbumin mouse model of asthma. Trachea from naïve or ovalbumin-sensitised and -challenged mice were compared for transcriptional, functional and proteomic differences using gene microarrays, ex vivo tracheal contraction, immunohistochemistry and Western blot analysis. FIZZ1 was expressed in ovalbumin-treated, but not naïve, trachea. Naïve trachea incubated with recombinant FIZZ1 exhibited denuded epithelium and contractile hyperresponsiveness. The FIZZ1-incubated trachea also exhibited an associated increased expression of phospho-c-Raf, phospho-extracellular signal-regulated kinase 1/2, phospho-p38, MLCK and MLC-20. These data demonstrate that FIZZ1 regulates tracheal smooth muscle contraction through impairment of the epithelium and activation of the mitogen-activated protein kinase pathway in muscle.