RT Journal Article
SR Electronic
T1 In vivo and in vitro inactivation of bovine surfactant by an anti-surfactant monoclonal antibody
JF European Respiratory Journal
JO Eur Respir J
FD European Respiratory Society
SP 1245
OP 1250
VO 4
IS 10
A1 Eijking, EP
A1 Strayer, DS
A1 van Daal, GJ
A1 Tenbrinck, R
A1 Merritt, TA
A1 Hannappel, E
A1 Lachmann, B
YR 1991
UL http://erj.ersjournals.com/content/4/10/1245.abstract
AB In this study the importance of a low-weight surfactant protein (11 kDa) is demonstrated by selectively blocking this protein with a monoclonal antibody. In adult rats respiratory failure was induced by repeated bronchoalveolar lavage to remove all pulmonary surfactant. It was shown that surfactant mixed with the antibody was not capable of restoring lung function when compared with surfactant alone or surfactant mixed with control serum. Using the pulsating bubble surfactometer, it could be demonstrated that surfactant mixed with this antibody had a significant higher minimum surface tension when compared with surfactant alone, or surfactant mixed with an unrelated mouse immunoglobulin G (IgG). The inhibition of surfactant function by the monoclonal antibody suggests the importance of the 11 kDa protein for normal surfactant function.