RT Journal Article
SR Electronic
T1 Interleukin-1 receptor-related protein ST2 suppresses the initial stage of bleomycin-induced lung injury
JF European Respiratory Journal
JO Eur Respir J
FD European Respiratory Society
SP 1415
OP 1428
DO 10.1183/09031936.00084307
VO 33
IS 6
A1 N. Mato
A1 M. Fujii
A1 Y. Hakamata
A1 E. Kobayashi
A1 A. Sato
A1 M. Hayakawa
A1 H. Ohto-Ozaki
A1 M. Bando
A1 S. Ohno
A1 S. Tominaga
A1 Y. Sugiyama
YR 2009
UL http://erj.ersjournals.com/content/33/6/1415.abstract
AB Acute lung injury has a range of causes, and occasionally leads to lethal respiratory failure. Despite advances in treatment, acute lung injury continues to have a high mortality rate, and thus a new therapeutic approach is needed. ST2 is an interleukin (IL)-1 receptor-related protein, and its expression is induced by various inflammatory responses. Recently, ST2 has been speculated to exert anti-inflammatory effects; therefore, we investigated the role of the ST2 in the murine model of acute lung injury. To elucidate the function of ST2 in vivo, mice that transiently overexpressed ST2 protein were prepared using the hydrodynamic gene transfer method, and lung injury was induced by intratracheal administration of bleomycin. In bleomycin-treated ST2-overexpressing mice, the increase of neutrophils in the bronchoalveolar lavage fluid (BALF) was markedly suppressed. Additionally, the levels of tumour necrosis factor-α and IL-6, as well as the concentration of albumin, in BALF were reduced compared with those of controls. Furthermore, the pulmonary architecture in ST2-overexpressing mice remained almost normal, and the survival rate was significantly improved. From these results, we concluded that ST2 has the potential to suppress the initial stage of acute lung injury, and therefore it may be a useful reagent for the treatment of acute lung injury.