RT Journal Article
SR Electronic
T1 <em>Interleukin 13</em>, <em>CD14</em>, pet and tobacco smoke influence atopy in three Dutch cohorts: the allergenic study
JF European Respiratory Journal
JO Eur Respir J
FD European Respiratory Society
SP 593
OP 602
DO 10.1183/09031936.00162407
VO 32
IS 3
A1 R. W. B. Bottema
A1 N. E. Reijmerink
A1 M. Kerkhof
A1 G. H. Koppelman
A1 F. F. Stelma
A1 J. Gerritsen
A1 C. Thijs
A1 B. Brunekreef
A1 C. P. van Schayck
A1 D. S. Postma
YR 2008
UL http://erj.ersjournals.com/content/32/3/593.abstract
AB Studying gene–environment interactions may elucidate the complex origins of atopic diseases but requires large study populations. Pooling data from several cohort studies may help but may also obscure findings. Gene–environment interactions in atopy development were studied and the benefits of pooling data were evaluated. Haplotype-tagging polymorphisms in the genes interleukin (IL)13 and CD14 were genotyped in 3,062 children from the following birth cohorts: the Prevention and Incidence of Asthma and Mite Allergy (PIAMA) study; the Prevention of Asthma in Children (PREVASC) study; and the Child, Parent, Health, Focus on Lifestyle and Predisposition (KOALA) study, and tested for association with total and specific immunoglobulin (Ig)E and interaction with tobacco smoke and pet exposure at ages 1, 2, 4 and 8 yrs by analysis of variance, Chi-squared tests and regression analyses. At all ages, in IL13, minor alleles of rs1295685 and rs20541 were significantly associated with elevated IgE levels in pooled analyses. In CD14, the rs2569190-TT and rs2569191-CC genotypes associated with lower IgE and decreased risk of sensitisation at 4 and 8 yrs in children exposed to pets, with an opposite effect in nonexposed children. Findings for IL13 and CD14 were comparable in separate cohorts. The present study indicates that atopy is importantly influenced by interleukin 13 at age 1–8 yrs and by CD14 in interaction with pet exposure at ages 4 and 8 yrs. Additionally, pooled data improved effect estimates and genetic effects could be detected in interaction with important environmental factors.