RT Journal Article
SR Electronic
T1 Evaluating the potential of IP-10 and MCP-2 as biomarkers for the diagnosis of tuberculosis
JF European Respiratory Journal
JO Eur Respir J
FD European Respiratory Society
SP 1607
OP 1615
DO 10.1183/09031936.00055508
VO 32
IS 6
A1 M. Ruhwald
A1 T. Bodmer
A1 C. Maier
A1 M. Jepsen
A1 M. B. Haaland
A1 J. Eugen-Olsen
A1 P. Ravn
A1 on behalf of TBNET
YR 2008
UL http://erj.ersjournals.com/content/32/6/1607.abstract
AB The aim of the present study was to evaluate the potential of diagnostic tests based on interferon-γ inducible protein (IP)-10 and monocyte chemotactic protein (MCP)-2, and compare the performance with the QuantiFERON TB® Gold In-Tube (QFT-IT; Cellestis, Carnagie, Australia) test. IP-10 and MCP-2 were determined in supernatants from whole blood stimulated with Mycobacterium tuberculosis-specific antigens. Samples were obtained from 80 patients with culture- and/or PCR-proven tuberculosis (TB), and 124 unexposed healthy controls: 86 high school students and 38 high school staff. IP-10 and MCP-2 test cut-offs were established based on receiver operating characteristic curve analysis. TB patients produced significantly higher levels (median) of IP-10 (2158 pg·mL−1) and MCP-2 (379 pg·mL−1) compared with interferon (IFN)-γ (215 pg·mL−1). The QFT-IT, IP-10 and MCP-2 tests detected 81, 83 and 71% of the TB patients; 0, 3 and 0% of the high school students and 0, 16 and 3% of the staff, respectively. Agreement between tests was high (>89%). By combining IP-10 and IFN-γ tests, the detection rate increased among TB patients to 90% without a significant increase in positive responders among the students. In conclusion, interferon-γ inducible protein-10 and monocyte chemotactic protein-2 responses to Mycobacterium tuberculosis-specific antigens could be used to diagnose infection. Combining interferon-γ inducible protein-10 and interferon-γ may be a simple approach to increase the detection rate of the Mycobacterium tuberculosis-specific in vitro tests.