PT - JOURNAL ARTICLE AU - M. Rumelhard AU - K. Ramgolam AU - R. Hamel AU - F. Marano AU - A. Baeza-Squiban TI - Expression and role of EGFR ligands induced in airway cells by PM<sub>2.5</sub> and its components AID - 10.1183/09031936.00085907 DP - 2007 Dec 01 TA - European Respiratory Journal PG - 1064--1073 VI - 30 IP - 6 4099 - http://erj.ersjournals.com/content/30/6/1064.short 4100 - http://erj.ersjournals.com/content/30/6/1064.full SO - Eur Respir J2007 Dec 01; 30 AB - The aim of the current study was to establish the epidermal growth factor receptor (EGFR) ligand expression profile in human airway epithelial cells exposed to either particulate matter (PM) with an aerodynamic diameter &lt;2.5 μm (PM2.5) or its components and the involvement of EGFR ligands in PM2.5-provoked airway inflammation. EGFR ligand mRNA and protein expression were studied in a human bronchial epithelial cell line and normal nasal cells exposed to noncytotoxic concentrations of PM2.5 or its components. The autocrine role of EGFR ligands in airway epithelial cell pro-inflammation was determined by adding conditioned media from PM2.5-treated cells to fresh cells and measuring the secretion of granulocyte-macrophage colony-stimulating factor (GM-CSF), a pro-inflammatory biomarker. PM2.5 increased amphiregulin, transforming growth factor-α and heparin-binding EGF-like growth factor mRNA expression and protein secretion, with a slight contribution of aqueous metallic compounds and a strong participation of organic components putatively attributed to PM polyaromatic hydrocarbon content. PM2.5-induced EGFR ligands were involved in cellular GM-CSF release. The current study revealed upregulation of several epidermal growth factor receptor ligands by airway epithelial cells exposed to particulate matter with an aerodynamic diameter &lt;2.5 μm and their contribution to bronchial epithelial cell granulocyte-macrophage colony-stimulating factor secretion by an autocrine action, suggesting that these ligands could elicit and sustain the particulate matter-induced airway pro-inflammatory response and contribute to bronchial remodelling.