TY - JOUR T1 - Keratinocyte growth factor improves alterations of lung permeability and bronchial epithelium in allergic rats JF - European Respiratory Journal JO - Eur Respir J SP - 31 LP - 39 DO - 10.1183/09031936.00011606 VL - 30 IS - 1 AU - I. Tillie-Leblond AU - P. Gosset AU - R. Le Berre AU - A. Janin AU - T. Prangère AU - A. B. Tonnel AU - B. P. H. Guery Y1 - 2007/07/01 UR - http://erj.ersjournals.com/content/30/1/31.abstract N2 - Chronic allergic asthma is associated with marked inflammatory reaction, microvascular leakage and epithelium injury. As previously shown in a rat model of chronic asthma, these alterations increase lung permeability and distal airway fluid clearance. Keratinocyte growth factor (KGF) has been shown to induce epithelial cell proliferation and to protect from acute lung injuries. Therefore, the current authors evaluated the potential role of KGF treatment on lung permeability and airway inflammation in rats with chronic asthma. KGF (1 mg·kg−1) was administered intravenously before the last ovalbumin (OVA) challenge in sensitised rats. Permeability was assessed by the leak of radiolabelled albumin from the alveolar and systemic compartments. Histopathological analysis was also performed. Treatment with KGF decreased the leak of both markers and decreased the level of extravascular lung water in sensitised rats challenged with OVA. KGF treatment also reduced the inflammatory cell number in bronchoalveolar lavage fluid but not in bronchial mucosa. KGF markedly limited the allergen-induced alterations in epithelium integrity and the expression of the intercellular junction proteins β-catenin and zonula occludens protein-1. In conclusion, keratinocyte growth factor administration markedly limits lung permeability and airway inflammation, an effect associated with a decrease in epithelium alterations during chronic allergic asthma. These data open new prospects in the therapeutic strategy of asthma. ER -