RT Journal Article
SR Electronic
T1 Telomerase activation in a model of lung adenocarcinoma
JF European Respiratory Journal
JO Eur Respir J
FD European Respiratory Society
SP 1175
OP 1182
DO 10.1183/09031936.06.00125105
VO 27
IS 6
A1 F. Suau
A1 V. Cottin
A1 F. Archer
A1 S. Croze
A1 J. Chastang
A1 G. Cordier
A1 F. Thivolet-Béjui
A1 J-F. Mornex
A1 C. Leroux
YR 2006
UL http://erj.ersjournals.com/content/27/6/1175.abstract
AB Ovine pulmonary adenocarcinoma (OPA) is a lung cancer strikingly similar to the pneumonic-type mixed invasive adenocarcinoma with a predominant bronchioloalveolar component in humans. Telomerase activity in OPA and the potential involvement of the kinase Akt in telomerase activation and regulation of cell proliferation were investigated. Lung tissues were collected from sheep with a histopathological diagnosis of OPA or controls. Epithelial cell cultures were derived in vitro from lung tissues. Telomerase activity was evaluated using the telomeric repeat amplification protocol method. Phosphorylation of Akt was detected by Western blotting. Telomerase activity was significantly higher in OPA lung tissues compared to control lung tissues. A high telomerase activity was detected in eight out of 12 (67%) primary cell cultures derived from tumours. A high level of expression of phosphorylated Akt was found in 10 out of 27 (37%) tumours, with abolition of Akt activation in response to epidermal growth factor stimulation demonstrated in primary cell cultures derived from tumours. Telomerase activation takes place in ovine pulmonary adenocarcinoma tumour cells and may be partly attributable to Akt activation. Telomerase may inhibit cellular senescence and contribute to the accumulation of tumour cells in mixed adenocarcinoma with a bronchioloalveolar component. Further work is necessary to identify alternative signalling pathways of telomerase activation in tumours.