RT Journal Article
SR Electronic
T1 Decreased macrophage release of TGF-β and TIMP-1 in chronic obstructive pulmonary disease
JF European Respiratory Journal
JO Eur Respir J
FD European Respiratory Society
SP 60
OP 66
DO 10.1183/09031936.05.00045504
VO 26
IS 1
A1 A. R. Pons
A1 J. Sauleda
A1 A. Noguera
A1 J. Pons
A1 B. Barceló
A1 A. Fuster
A1 A. G. N. Agustí
YR 2005
UL http://erj.ersjournals.com/content/26/1/60.abstract
AB The present study tested the hypothesis that alveolar macrophages (AM) from patients with chronic obstructive pulmonary disease (COPD) release more pro-inflammatory and/or less anti-inflammatory mediators than those from smokers with normal lung function and never-smokers. AM were sorted by flow cytometry from bronchoalveolar lavage fluid in 13 patients with COPD (mean±SEM 67±2 yrs, forced expiratory volume in one second (FEV1) 61±4% reference), 16 smokers with normal lung function (55±2 yrs, FEV1 97±4% reference) and seven never-smokers (67±7 yrs, FEV1 94±4% reference). After sorting, AM were cultured (with and without lipopolysaccharide stimulation) after 4 h and 24 h, and the concentrations of leukotriene B4 (LTB4), transforming growth factor (TGF)-β1 and tissue inhibitor of metalloproteinase (TIMP)-1 were quantified in the supernatant by ELISA. The production of reactive oxygen intermediates (ROI) in freshly isolated AM was determined by flow cytometry. LTB4 secretion and ROI production were not different between groups. In contrast, AM from COPD patients released significantly less TGF-β1 and TIMP-1 than those from smokers with normal lung function and nonsmokers. In conclusion, these observations are compatible with reduced anti-inflammatory and anti-elastolytic capacity in chronic obstructive pulmonary disease, which is likely to contribute to the pathogenesis of the disease.