RT Journal Article
SR Electronic
T1 Primary airway epithelial cell culture from lung transplant recipients
JF European Respiratory Journal
JO Eur Respir J
FD European Respiratory Society
SP 1080
OP 1085
DO 10.1183/09031936.05.00141404
VO 26
IS 6
A1 I. A. Forrest
A1 D. M. Murphy
A1 C. Ward
A1 D. Jones
A1 G. E. Johnson
A1 L. Archer
A1 F. K. Gould
A1 T. E. Cawston
A1 J. L. Lordan
A1 P. A. Corris
YR 2005
UL http://erj.ersjournals.com/content/26/6/1080.abstract
AB Long-term survival in lung transplantation is limited by the development of obliterative bronchiolitis, a condition characterised by inflammation, epithelial injury, fibroproliferation and obliteration of bronchioles leading to airflow obstruction. To investigate the role of the bronchial epithelium in the pathogenesis of obliterative bronchiolitis the current study aimed to establish primary bronchial epithelial cell cultures (PBEC) from lung allografts. Four to six bronchial brushings were obtained from sub-segmental bronchi of lung allografts. Cells were seeded onto collagen-coated plates and grown to confluence in bronchial epithelial growth medium. Bronchial brushings (n = 33) were obtained from 27 patients. PBECs were grown to confluence from 12 out of 33 (39%) brushings. Failure to reach confluence was due to early innate infection. Bacteria were usually isolated from both bronchoalveolar lavage and culture media, but a separate population was identified in culture media only. Primary culture of bronchial epithelial cells from lung transplant recipients is feasible, despite a high rate of early, patient-derived infection. Latent infection of the allograft, identified only by bronchial brushings, may itself be a persistent stimulus for epithelial injury. This technique facilitates future mechanistic studies of airway epithelial responses in the pathogenesis of obliterative bronchiolitis.