PT  - JOURNAL ARTICLE
AU  - T. Imaizumi
AU  - M. Kumagai
AU  - K. Taima
AU  - T. Fujita
AU  - H. Yoshida
AU  - K. Satoh
TI  - Involvement of retinoic acid-inducible gene-I in the IFN-γ/STAT1 signalling pathway in BEAS-2B cells
AID  - 10.1183/09031936.05.00102104
DP  - 2005 Jun 01
TA  - European Respiratory Journal
PG  - 1077--1083
VI  - 25
IP  - 6
4099  - http://erj.ersjournals.com/content/25/6/1077.short
4100  - http://erj.ersjournals.com/content/25/6/1077.full
SO  - Eur Respir J2005 Jun 01; 25
AB  - Bronchial epithelial cells play an important role in airway host defence, and interferon (IFN)-γ controls immune reactions by regulating the expression of various genes in bronchial epithelial cells. Signal transducer and activator of transcription 1 (STAT1) is the key transcriptional factor in IFN-γ signalling. Retinoic acid-inducible gene-I (RIG-I) is a member of the DExH box family of proteins and designated a putative RNA helicase. RNA helicases play diverse roles in regulation of gene expression and cellular functions, and RIG-I is implicated in antiviral responses. The aim of the present study was to investigate the effect of IFN-γ on RIG-I expression in a cell line derived from human bronchial epithelial cells, BEAS-2B. Induction of RIG-I in response to IFN-γ was found in BEAS-2B cells. Induction of RIG-I by IFN-γ was also demonstrated in another pulmonary epithelial cell line, NCI-H292. Transfection of BEAS-2B cells with RIG-I complementary DNA resulted in the upregulation of STAT1. Induction of IFN-γ-inducible protein 10 by IFN-γ was enhanced in the cells overexpressing RIG-I. It is concluded that retinoic acid-inducible gene-I may play an important role in the regulation of immunological reactions in bronchial epithelial cells elicited by interferon-γ.