TY - JOUR T1 - Induction of type-IIA secretory phospholipase A2 in animal models of acute lung injury JF - European Respiratory Journal JO - Eur Respir J SP - 1040 LP - 1045 DO - 10.1183/09031936.03.00093002 VL - 21 IS - 6 AU - H.L. Attalah AU - Y. Wu AU - M. Alaoui-El-Azher AU - F. Thouron AU - K. Koumanov AU - C. Wolf AU - L. Brochard AU - A. Harf AU - C. Delclaux AU - L. Touqui Y1 - 2003/06/01 UR - http://erj.ersjournals.com/content/21/6/1040.abstract N2 - The aim of this study was to evaluate the presence of type-II secretory phospholipase A2 (sPLA2-IIA) in alveolar space and its possible role in the destruction of surfactant in three rat models of acute lung injury. Alveolar instillation of either lipopolysaccaride or live Pseudomonas aeruginosa resulted in a significant increase in lung oedema and in a decrease in static compliance of the respiratory system together with alveolar-neutrophil influx as compared with healthy control rats. The upregulation of messenger ribonucleic acid and sPLA2-IIA by the lung was evident. This was associated with surfactant degradation and a decrease in large:small ratio of surfactant aggregates in bacteria-instilled rats. A negative correlation between compliance and sPLA2-IIA activity in bronchoalveolar lavage fluid was shown. By contrast, during alpha naphthylthiourea-induced injury, neither alveolar-neutrophil influx nor increase in sPLA2-IIA activity was observed. Additional experiments in rats treated with a specific inhibitor of type-II secretory phospholipase A2 activity (3 acetamine-1-benzyl-2 ethylindolyl-5 oxy; propane phosphonic acid (LY311727)) demonstrated no improvement in physiological parameters despite a biochemical effect, suggesting that its activity is only one of the multiple factors involved in the pathophysiology of lung injury. ER -