@article {Peters946, author = {MJ Peters and AB Breslin and N Berend}, title = {The effect of anticholinergic and beta-agonist pretreatment on bronchoconstriction induced by N-formyl-methionyl-leucyl-phenylalanine}, volume = {2}, number = {10}, pages = {946--949}, year = {1989}, doi = {10.1183/09031936.93.02100946}, publisher = {European Respiratory Society}, abstract = {N-Formyl-methionyl-leucyl-phenylalanine (FMLP), an acylated tripeptide bacterial neutrophil chemotactic factor, has been shown to be a bronchoconstrictor by inhalation in man in vivo. It thus has a putative role in the generation of bronchoconstriction associated with bacterial bronchial infection. We have investigated the effect of pretreatment with the anticholinergic agent, ipratropium bromide (IB), and the beta 2-agonist, fenoterol (F), on FMLP-induced bronchoconstriction. Ten non-asthmatic subjects aged 21-28 yrs performed dose-response curves to nebulized FMLP on 3 study days after pretreatment with saline, F or IB. Amongst the 8 subjects who bronchoconstricted by 20\% to FMLP there was a significant increase in the provocative concentration causing a 20\% fall in forced expiratory volume in one second (FEV1) (PC20FMLP) after both IB and F. F was significantly better than IB. When comparison was made using absolute fall in FEV1 and including all 10 subjects the same results were found. Partial inhibition of FMLP-induced bronchoconstriction by IB suggests that part of the effect of FMLP is vagally mediated. We suggest that F is acting via modulation of FMLP-induced rises in intracellular free calcium.}, issn = {0903-1936}, URL = {https://erj.ersjournals.com/content/2/10/946}, eprint = {https://erj.ersjournals.com/content/2/10/946.full.pdf}, journal = {European Respiratory Journal} }