RT Journal Article
SR Electronic
T1 Murine strain differences in airway inflammation caused by diesel exhaust particles
JF European Respiratory Journal
JO Eur Respir J
FD European Respiratory Society
SP 291
OP 298
DO 10.1183/09031936.98.11020291
VO 11
IS 2
A1 Y Miyabara
A1 R Yanagisawa
A1 N Shimojo
A1 H Takano
A1 HB Lim
A1 T Ichinose
A1 M Sagai
YR 1998
UL http://erj.ersjournals.com/content/11/2/291.abstract
AB To elucidate whether immunoglobulin (Ig) E or IgG are involved in the murine asthma model, we compared the pathogenic features of mice that were high IgG responders (C3H/He) with mice that were high IgE responders (BALB/c) after intratracheal instillation of diesel exhaust particles (DEP) and ovalbumin sensitization. Both mouse strains received DEP intratracheally once a week for 5 weeks. After the second injection of DEP, ovalbumin and aluminium hydroxide were injected intraperitoneally. After the last DEP administration, the mice were challenged by exposure to an aerosol of ovalbumin. DEP caused increased IgG1 production and airway hyperresponsiveness after ovalbumin sensitization in C3H/He mice, although IgE production did not change in either strain. Furthermore, in C3H/He mice, the number of eosinophils and goblet cells in the bronchial epithelium, and the expression of interleukin-5 and interleukin-2 were increased by DEP and ovalbumin treatments. In contrast, the pathogenic changes in BALB/c mice were weak, even though the same protocol was used. In conclusion, murine strain differences in response to air pollutants and allergens seem to be related to antigen-specific immunoglobulin G1 production and cytokine expression in the lungs.