PT  - JOURNAL ARTICLE
AU  - Wang, Jiun-Long
AU  - Chen, Chuan-Mu
TI  - Crosstalk between Metformin and angiogenesis in lung cancer model
AID  - 10.1183/13993003.congress-2023.PA2205
DP  - 2023 Sep 09
TA  - European Respiratory Journal
PG  - PA2205
VI  - 62
IP  - suppl 67
4099  - https://publications.ersnet.org//content/62/suppl_67/PA2205.short
4100  - https://publications.ersnet.org//content/62/suppl_67/PA2205.full
SO  - Eur Respir J2023 Sep 09; 62
AB  - Purpose:    Reposition of Metformin as anti-tumor effect is well discussed among different cancer types including lung cancer. We attempt to explore anti-tumorigenesis pathway exerted by metformin in lung cancer management. We utilize the GSEA (Gene Set Enrichment Analysis ) as a useful tool for further approach.   Materials and Method:   First, we designed lung cancer cell line (A549) exposure to metformin under normoxia or hypoxia status. Second, we harvested the cell line post treatment and extracted RNA and transfer to cDNA gradually. We analyzed the cDNA microarray by means of Gene Study Enrichment Analysis (GSEA) method. Among anti-angiogenesis and micro-environment engaged in lung cancer development and treatment were explored.Results:   Preliminary we find the metformin possess the anti-angiogenesis function by suppression hypoxia induced factor(HIF)-1α and PGK1 expression and proved by microarray (heat map) and western blot analysis. Further in vivo study is anticipated to elucidate the anti-tumor effect of metformin.   Conclusion:    We found Metformin can counteract the angiogenesis pathway, especially when exposure to hypoxia condition. Further approach the signal transduction and real mechanism between metformin and hypoxia environment involved in the anti-tumorigenesis needs further investigation.FootnotesCite this article as: European Respiratory Journal 2023; 62: Suppl. 67, PA2205.This abstract was presented at the 2023 ERS International Congress, in session “Inflammatory endotyping: the macrophage across disease areas”.This is an ERS International Congress abstract. No full-text version is available. Further material to accompany this abstract may be available at www.ers-education.org (ERS member access only).