RT Journal Article SR Electronic T1 SPLUNC1 in sputum of children with cystic fibrosis pulmonary exacerbations JF European Respiratory Journal JO Eur Respir J FD European Respiratory Society SP PA4996 DO 10.1183/13993003.congress-2023.PA4996 VO 62 IS suppl 67 A1 Ben-Meir, Elad A1 Perrem, Lucy A1 Ratjen, Felix A1 Grasemann, Hartmut YR 2023 UL https://publications.ersnet.org//content/62/suppl_67/PA4996.abstract AB Background: SPLUNC1 is an innate defense protein that acts as anti-microbial agent and regulates airway surface liquid volume. We aimed to investigate the role of sputum SPLUNC1 as a biomarker for pulmonary exacerbations (PEx) in children with cystic fibrosis (CF).Methods: Sputum samples from CF children with PEx treated with i) intravenous antibiotic therapy for severe PEx, or ii) oral antibiotics for mild PEx, as well as from the TRACK study (Perrem, L et al., AJRCCM 2021; 203(8):977-86), in which CF school-age children were followed prospectively for two years. SPLUNC1, interleukin-8 (IL-8) and neutrophil elastase (NE) were measured by ELISA. Paired analyses were performed by Wilcoxon-test.Results: Eleven CF children with a median (range) age of 13.5 (9.6-17.0) years were treated for severe PEx. Median SPLUNC1 levels were 529.3 (224.6-1698) before and 2705 (642.1-3975) ng/mL after treatment (p=0.003). Seven children, 11.38 (8.4-16.8) years of age, were treated for mild PEx. SPLUNC1 increased from 189.5 (97.3-314.9) before to 362.5 (220.5-1015.0) ng/mL after treatment (p=0.031). In TRACK, there were 17 participants with samples from a stable visit followed by a PEx visit after 147 (24-378) days and 21 with samples from a PEx followed by a stable visit after 176 (50-430) days. SPLUNC1 decreased from 3101 (623.7-4913) at stable visit to 1515 (512.8-4424) ng/mL at PEx (p=0.035) and increased from 1390 (505.4-1520) at PEx to 3482 (1820-6179) ng/mL at stable follow-up (p=0.001). There were no correlations between SPLUNC1 and IL-8 or NE levels in sputum, FEV1 or the lung clearance index (LCI).Conclusion: SPLUNC1 sputum levels are decreased in PEx and have promising clinimetric properties as a biomarker in CF children.FootnotesCite this article as: European Respiratory Journal 2023; 62: Suppl. 67, PA4996.This abstract was presented at the 2023 ERS International Congress, in session “Inflammatory endotyping: the macrophage across disease areas”.This is an ERS International Congress abstract. No full-text version is available. Further material to accompany this abstract may be available at www.ers-education.org (ERS member access only).