RT Journal Article
SR Electronic
T1 Environmental nanoparticle exposure triggers gammaherpesvirus reactivation via the MAPK signaling pathway in macrophages
JF European Respiratory Journal
JO Eur Respir J
FD European Respiratory Society
SP 4576
DO 10.1183/13993003.congress-2022.4576
VO 60
IS suppl 66
A1 Han, L
A1 Haefner, V
A1 Kutschke, D
A1 Steer, B
A1 Fuchs, A
A1 Irmler, M
A1 Beckers, J
A1 Feuchtinger, A
A1 Heier, M
A1 Peters, A
A1 Adler, H
A1 Stoeger, T
YR 2022
UL http://erj.ersjournals.com/content/60/suppl_66/4576.abstract
AB Environmental particle inhalation and persistent herpesvirus infection are omnipresent and associated with chronic lung diseases. Previously, we showed that pulmonary exposure to soot-like carbonaceous nanoparticles (CNP) or fiber-shaped engineered double walled carbon nanotubes (DWCNT) induced lytic viral protein expression increase in latently murine gammaherpesvirus-68 (MHV-68) infected mouse lungs, with similar pattern as acute infection suggesting virus reactivation. However, the association between particle exposure and human herpesvirus reactivation and the underlying mechanisms remain unclear.In the KORA cohort study, we found Human Herpesvirus 6 (HHV6) antibody titers are associated with high particle number concentration (PNC). In MHV-68 infected murine model, CNP exposure reactivated herpesvirus mainly localized to CD11b+ infiltrating macrophages. Mechanistically, ERK1/2, JNK and p38 were rapidly activated within the first hour after NPs exposure in persistently MHV-68 infected bone marrow derived macrophages (Ana-1/MHV-68), followed by lytic viral gene expression upregulation and viral titer increase. Further, pharmacological inhibition of p38 activation abrogated CNP but not DWCNT triggered virus reactivation. In vivo, p38 inhibitor pretreatment in latently infected mice attenuates MHV-68 reactivation induced by CNP exposure.Our findings suggest that environmental particle exposure triggers herpesvirus reactivation in the lung via p38 MAPK dependent signaling, and pharmacological inhibition of p38 might alleviate ambient particle exposure related disease exacerbations.FootnotesCite this article as Eur Respir J 2022; 60: Suppl. 66, 4576.This article was presented at the 2022 ERS International Congress, in session “-”.This is an ERS International Congress abstract. No full-text version is available. Further material to accompany this abstract may be available at www.ers-education.org (ERS member access only).